検査と技術38巻10号

2010年09月発行

増刊号 免疫反応と臨床検査2010 I　総論―免疫反応の基礎 A　イムノアッセイの変遷

イムノアッセイの変遷

著者： 芦原義弘¹

所属機関： ¹富士レビオ株式会社研究部門

ページ範囲：P.748 - P.756

はじめに

　イムノアッセイ(immunoassay，免疫測定法)が実際の臨床検査に利用されてから既に50年以上が過ぎ，それまで多くの技術革新がみられた．特に放射免疫測定(radioimmunoassay，RIA)法から非RIA法への技術革新は，臨床検査現場における自由度や実用面で大きな貢献があったといえる．

　本稿では，特にRIA法の実用化から最近の非RIA法自動化システムの流れを中心に，イムノアッセイの変遷を報告する．

参考文献

1) Heidelberger M, Kendall FE:A quantitative study of the precipitin reaction between type III Pneumococcus polysaccharide and purified homologous antibody. J Exp Med30：809-823，1929

2) Fahey JL, McKelvey EM:Quantification determination of serum immunoglobulins in antibody-agar plates. J Immunol94：84-90，1965

3) Boyden SV:The absorption of protein on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med93：107-120，1951

4) Ito T, Kuwahara S, Yokota T:Automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of Escherichia coli. J Clin Microbiol17：7-12，1983

5) Masson PL, Holy HW:Immunoassay by particle counting. Rose NR, Friedman H, Fahey JL, et al (eds):Manual of Clinical Labolatory Immunology, 3rd ed. American Society for Microbiology Washington DC，pp43-48，1986

6) Yallow RS, Berson SA:Assay of plasma insulin in human subjects by immunological methods. Nature184：1648-1649，1959

7) Nakamura RM, Kasahara Y:Heterogeneous enzyme immunoassays. Nakamura RM, Kasahara Y, Rechnitz GA (eds):Immunochemical Assays and Biosensor Techonology for the 1990s. American Society for Micryobiology, Washington DC，pp149-167，1992

8) Kitagawa T, Fujitake T, Taniyama H, et al:Enzyme immunoassay of viomycin, new cross-linking reagent for enzyme-labelling and a preparation method for antiserum of viomycin. J Biochem83：1493-1501，1978

9) Nakamura RM:Fluorescence immunoassays. Nakamura RM, Kasahara Y, Rechnitz GA (eds):Immunochemical Assays and Biosensor Technology for the 1990s. American Society for Microbiology, Washington DC，pp205-227，1992

10) Bounaud MP, Bounara JY, Bouin-Pineau MH, et al:Chemiluminescence immunoassay of thyrotropin with acridinium-ester-labeled antibody evaluated and compared with two other immunoassays. Clin Chem33：2096-2100，1987

11) Kasahara Y:Homogeneous enzyme immunoassays. Nakamura RM, Kasahara Y, Rechnitz GA (eds):Immunochemical Assays and Biosensor Technology for the 1990s. American Society for Microbiology, Washington DC，pp169-182，1992

12) Rubenstein KE, Schneider RS, Ullman EF:Homogeneous enzyme immunoassay;A new immunological technique. Biochim Biophys Res Commun47：846-851，1972

13) Boyd JC, Savory MG, Margrey M, et al:Adaptation of EMIT drug assays to a random-access automated clinical analyzer. Ann Clin Lab Sci15：39-44，1985

14) Dandliker WB, de Saussure VA:Fluorescence polarization in immunochemistry. Immunochemistry7：799-828，1970

15) Ashihara Y, Nishizono I, Miyagawa E, et al:Homogeneous enzyme immunoassay for high molecular weight antigen (I). J Clin Lab Anal2：138-142，1988

16) Ashihara Y, Hiraoka T, Makino Y, et al:Immunoassay for determining low- and high-Mr antigens with a dry multilayer film. Clin Chem37：1525-1526，1991

17) Engvall E, Perlman P:Enzyme-linked immunosorbent assay (ELISA);Quantitative assay of immunoglobulin G. Immunochemistry8：871-874，1971

18) Volier A, Bidwell D, Bartlett A:Enzyme immunoassays in diagnostic medicine. Bull WHO53：55-65，1976

19) Nishizono I, Iida S, Suzuki N, et al:Rapid and sensitive chemiluminescent enzyme immunoassay for measuring tumor markers. Clin Chem37：1639-1644，1991

20) Weeks I, Beheahti I, McCapra F, et al:Acridinium esters as high specific activity labels in immunoassay. Clin Chem29：1274-1279，1983

21) Bounaud MP, Bounara JY, Bouin-Pineau MH, et al:Chemiluminescence immunoassay of thyrotropin with acridinium-ester-labeled antibody evaluated and compared with two other immunoassays. Clin Chem33：2096-2100，1987

22) Thorpe GH, Kricka LJ, Moseley SB, et al:Phenols as enhancers of the chemiluminescent horseradish peroxidase-luminol-hydrogen peroxide reaction;Application in luminescence- monitored enzyme immunoassays. Clin Chem31：1335-1341，1985

23) Bronstein I:Method of detecting qa substance enzymatically induced decomposition of dioxetanes PCT/US87/00554 (WO88/00695), 1988

24) Bronstein I, Voyta J, Thorpe G, et al:Chemiluminescent assay of alkaline phosphatase applied in an ultra-sensitive enzyme immunoassay of thyrotropin. Clin Chem35：1441-1446，1989

25) Blackburn GF, Shah HP, Kenten JH, et al:Electrochemiluminescence detection for development of immunoassays and DNA probe assays for clinical diagnostics. Clin Chem37：1534-1539，1991

26) Minekawa T, Ohkuma H, Abe K, et al:Development of ultra-high sensitivity bioluminescent enzyme immunoassay for hepatitis B virus surface antigen using firefly luciferase. Luminescence24：394-399，2009

27) Wu AH, Wong SS, Waldron C, et al:Quantification of choriogonadotropin;Analytical correlation between serum and urine with creatinine correction. Clin Chem33：1424-1426，1987

28) 鎌田智：全自動エンザイムイムノアッセイ装置AIA1200．Laboratory and Clinical Practice5：61-63，1987

29) 芦原義弘，櫻林恭介，岡田政久：全自動化学発光酵素免疫システム全自動CLEIA．Current Laboratory Medicine9：186-188，1991

30) Isomura M, Ueno M, Shimada K, et al:Highly sensitive chemiluminescent enzyme immunoassay with gelatin-coated ferrite solid phase. Clin Chem40：1830，1994

31) Sturenburg E, Junker R:Point-of-care testing in microbiology;The advantages and disadvantages of immunochromatographic test strips. Dtsch Arztebl Int106：48-54，2009

32) Kasahara Y, Ashihara Y:Simple devices and their possible application in clinical laboratory downsizing. Clin Chim Acta267：87-102，1997

33) Apple FS, Christenson RH, Valdes R Jr, et al:Simultaneous rapid measurement of whole blood myoglobin, creatine kinase MB, and cardiac troponin I by the triage cardiac panel for detection of myocardial infarction. Clin Chem45：199-205，1999

34) 井上賢二，豊田尚子，東谷高徳，他：全自動蛍光免疫測定装置「ミュータスワコーi30」の基礎検討．日本臨床検査自動化学会誌34(抄録集)：691，2009

35) Himmelhaus M, Francois A:In-vitro sensing of biomechanical forces in live cells by a whispering gallery mode biosensor. Biosens Bioelectron25：418-427，2009