臨床眼科70巻11号

2016年10月発行

増刊号 眼感染症の傾向と対策—完全マニュアル Ⅰ．知っておきたい眼感染症診療の動向

角膜ヘルペスの診断，治療の歴史

著者： 檜垣史郎¹

所属機関： ¹若弘会若草第一病院眼科

ページ範囲：P.23 - P.27

POINT

■1972年に提唱された角膜ヘルペス病型分類，および，1993年に提唱された角膜ヘルペス病型分類について述べた。

■real-time PCR法，multiplex real-time PCR法，LAMP法などについて概説した。

■単純ヘルペスウイルスに有効と期待される薬剤について述べた。

参考文献

1）大橋裕一・石橋康久・井上幸次・他（眼ヘルペス感染症研究会）：角膜ヘルペス—新しい病型分類の提案．眼科37：759-764，1995

2）下村嘉一・大橋裕一・田川義継・他（眼ヘルペス感染症研究会）：上皮型角膜ヘルペスの新しい診断基準．眼科44：739-742，2002

3）Mullis KB, Faloona FA：Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol 155：335-350, 1987

4）Saiki RK, Scharf S, Faloona F et al：Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230：1350-1354, 1985

5）Crouse CA, Pflugfelder SC, Pereira I et al：Detection of herpes viral genomes in normal and diseased corneal epithelium. Curr Eye Res 9：569-581, 1990

6）Heid CA, Stevens J, Livak KJ et al：Real time quantitative PCR. Genome Res 6：986-994, 1996

7）Morozumi M, Nakayama E, Iwata S et al：Molecular beacon probe simultaneous detection of pathogens in clinical samples from patients with community-acquired pneumonia by real-time PCR with pathogen-specific molecular beacon probes. J Clin Microbiol 44：1440-1446, 2006

8）Bekkaoui F, Poisson I, Crosby W et al：Cycling probe technology with RNase H attached to an oligonucleotide. Biotechniques 20：240-248, 1996

9）Simpson DA, Feeney S, Boyle C et al：Retinal VEGF mRNA measured by SYBR green I fluorescence：A versatile approach to quantitative PCR. Mol Vis 5：178-183, 2000

10）Fukuda M, Deai T, Higaki S et al：Quantitative analysis of herpes simplex virus genome in tears from patients with herpetic keratitis. Cornea 22（Suppl）：S55-60, 2003

11）Kaufman HE, Azcuy AM, Varnell ED et al：HSV-1 DNA in tears and saliva of normal adults. Invest Ophthalmol Vis Sci 46：241-247, 2005

12）Kakimaru-Hasegawa A, Kuo CH, Komatsu N et al：Clinical application of real-time polymerase chain reaction for diagnosis of herpetic diseases of the anterior segment of the eye. Jpn J Ophthalmol 52：24-31, 2008

13）Higaki S, Itahashi M, Shimomura Y：Real-time PCR and multiplex real-time PCR for ocular infections by human herpes viruses. Acta Medica Kinki Univ 40：1-5, 2015

14）Kaneko H, Iida T, Aoki K et al：Loop-Mediated Isothermal Amplification法Sensitive and rapid detection of herpes simplex virus and varicella-zoster virus DNA by loop-mediated isothermal amplification. J Clin Microbiol 43：3290-3296, 2005

15）Inoue Y1, Shimomura Y, Fukuda M et al：Multicentre clinical study of the herpes simplex virus immunochromatographic assay kit for the diagnosis of herpetic epithelial keratitis. Br J Ophthalmol 97：1108-12, 2013

16）Brideau RJ, Knechtel ML, Huang A et al：Broad-spectrum antiviral activity of PNU-183792, a 4-oxo-dihydroquinoline, against human and animal herpesviruses. Antiviral Res 54：19-28, 2002

17）Kaufman HE1, Varnell ED, Gebhardt BM et al：Efficacy of a helicase-primase inhibitor in animal models of ocular herpes simplex virus type 1 infection. J Ocul Pharmacol Ther 24：34-42, 2008

18）Sasaki S, Miyazaki D, Haruki T et al：Efficacy of herpes virus helicase-primase inhibitor, ASP2151, for treating herpes simplex keratitis in mouse model. Br J Ophthalmol 97：498-503, 2013