臨床婦人科産科76巻4号

2022年04月発行

増刊号 最新の不妊診療がわかる！―生殖補助医療を中心とした新たな治療体系 第2章　生殖補助医療とその手技 体外受精―受精失敗を回避して，多くの良好受精卵を獲得する

顕微授精で受精卵の障害を低減するために―紡錘体の確認とPiezo-ICSI

著者： 奥山紀之¹ 京野廣一¹

所属機関： ¹京野アートクリニック高輪

ページ範囲：P.37 - P.40

▶紡錘体を観察することで減数分裂の状況を予測することができる．
▶Piezo-ICSIは変性率の低下などが期待できる低侵襲的な手法である．
▶安定した成績にはセッティングから温度管理までの厳密な培養室管理が必要である．

参考文献

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6）Hardarson T, et al : The position of the metaphase II spindle cannot be predicted by the location of the first polar body in the human oocyte. Hum Reprod 15 : 1372-1376, 2000

7）Taylor TH, et al : Effect of denuding on polar body position in in-vitro matured oocytes. Reprod Biomed Online 17 : 515-519, 2008

8）Rama Raju GA, et al : Meiotic spindle and zona pellucida characteristics as predictors of embryonic development : a preliminary study using PolScope imaging. Reprod Biomed Online 14 : 166-174, 2007

9）Montag M, et al : Spindle imaging in human oocytes : the impact of the meiotic cell cycle. Reprod Biomed Online 12 : 442-7446, 2006

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11）Tomari T, et al : Meiotic spindle size is a strong indicator of human oocyte quality. Reprod Med Biol 17 : 268-274, 2018

12）Hiraoka K, et al : Clinical efficiency of Piezo-ICSI using micropipettes with a wall thickness of 0.625μm. J Assist Reprod Genet 32 : 1827-1833, 2015

13）Takeuchi S, et al : Comparison of piezo-assisted micromanipulation with conventional micromanipulation for intracytoplasmic sperm injection into human oocytes. Gynecol Obstet Invest 52 : 158-162, 2001

14）Okuyama N, et al : Piezo-ICSI has clinical advantage to conventional ICSI by remarkably simple and stable technique that enables minimal damage on oocyte. P-128, ESHRE, 2019

15）Furuhashi K, et al : Piezo-assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years. Reprod Med Biol 18 : 357-361, 2019

16）Okuyama N, et al : The clinical results of piezo-ICSI compared to conventional-ICSI : a sibling-oocyte study. O-8, ASRM, 2019

17）Deirdre ZF, et al : PIEZO-ICSI increases fertilization rates compared with standard ICSI : a prospective cohort study. Reprod Biomed Online 43 : 404-412, 2021