臨床検査53巻1号

2009年01月発行

今月の主題 ウイルス感染症─最新の動向 各論 〈感染症ごとにみたウイルス感染症の診断と対策〉

ノロウイルス感染症

著者： 片山和彦¹

所属機関： ¹国立感染症研究所ウイルス第2部

ページ範囲：P.70 - P.76

　本稿では，世界中に広く分布し，年間数十万人から数百万人に及ぶ非細菌性急性胃腸炎患者を発生させ続けているノロウイルスの病態，ウイルスの性状，予防衛生について概説した．また，日進月歩の進歩を遂げる各種ノロウイルスの検査法は，感染の早期診断と汚染物質の隔離が有効なノロウイルスの感染予防に必須なアイテムである．超高感度核酸増幅法，簡便かつ迅速な診断法である抗原抗体反応に基づくELISA法，イムノクロマト法等の検査法に関して，有用性，将来の展望について概説した．

参考文献

1) Kapikian AZ, Wyatt RG, Dolin R, et al：Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol　10：1075-1081, 1972

2) Motomura K, Oka T, Yokoyama M, et al：Identification of monomorphic and divergent haplotypes in the 2006-2007 norovirus GII/4 epidemic population by genomewide tracing of evolutionary history. J Virol　82：11247-11262, 2008

3) Duizer E, Schwab KJ, Neill FH, et al：Laboratory efforts to cultivate noroviruses. J Gen Virol　85：79-87, 2004

4) Jiang X, Graham DY, Wang KN, et al：Norwalk virus genome cloning and characterization. Science　250：1580-1583, 1990

5) Prasad BV, Hardy ME, Dokland T, et al：X-ray crystallographic structure of the Norwalk virus capsid. Science　286：287-290, 1999

6) Vinje J, Koopmans MP：Simultaneous detection and genotyping of "Norwalk-like viruses" by oligonucleotide array in a reverse line blot hybridization format. J Clin Microbiol　38：2595-2601, 2000

7) Liu BL, Lambden PR, Gunther H, et al：Molecular characterization of a bovine enteric calicivirus：relationship to the Norwalk-like viruses. J Virol　73：819-825, 1999

8) Karst SM, Wobus CE, Lay M, et al：STAT1-dependent innate immunity to a Norwalk-like virus. Science　299：1575-1578, 2003

9) Kageyama T, Shinohara M, Uchida K, et al：Coexistence of multiple genotypes, including newly identified genotypes, in outbreaks of gastroenteritis due to Norovirus in Japan. J Clin Microbiol　42：2988-2995, 2004

10) Katayama K, Shirato-Horikoshi H, Kojima S, et al：Phylogenetic analysis of the complete genome of 18 Norwalk-like viruses. Virology　299：225-239, 2002

11) Teunis PF, Moe CL, Liu P, et al：Norwalk virus：how infectious is it? J Med Virol　80：1468-1476, 2008

12) Kageyama T, Kojima S, Shinohara M, et al：Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR. J Clin Microbiol　41：1548-1557, 2003

13) Kojima S, Kageyama T, Fukushi S, et al：Genogroup-specific PCR primers for detection of Norwalk-like viruses. J Virol Methods　100：107-114, 2002

14) Koopmans M, Vinje J, de Wit M, et al：Molecular epidemiology of human enteric caliciviruses in The Netherlands. J Infect Dis　181(Suppl 2)：S262-269, 2000

15) Kievits T, van Gemen B, van Strijp D, et al：NASBA isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection. J Virol Methods　35：273-286, 1991

16) Fukuda S, Sasaki Y, Takao S, et al：Recombinant norovirus implicated in gastroenteritis outbreaks in Hiroshima prefecture, Japan. J Med Virol　80：921-928, 2008

17) Masuda N, Yasukawa K, Isawa Y, et al：Rapid detection of tdh and trh mRNAs of Vibrio parahaemolyticus by the transcription-reverse transcription concerted(TRC)method. J Biosci Bioeng　98：236-243, 2004

18) Kamata K, Shinozaki K, Okada M, et al：Expression and antigenicity of virus-like particles of norovirus and their application for detection of noroviruses in stool samples. J Med Virol　76：129-136, 2005

19) Hansman GS, Natori K, Shirato-Horikoshi H, et al：Genetic and antigenic diversity among noroviruses. J Gen Virol　87：909-919, 2006

20) Tanaka T, Kitamoto N, Jiang X, et al：High efficiency cross-reactive monoclonal antibody production by oral immunization with recombinant norwalk virus-like particles. Microbiol Immunol　50：883-888, 2006

21) Mizutani T, Endoh D, Okamoto M, et al：Rapid genome sequence of RNA viruses. Emerg Infect Dis　13：322-324, 2007