臨床検査56巻12号

2012年11月発行

今月の主題 MDS(骨髄異形成症候群) 各論

MDSのimmunophenotyping

著者： 佐藤尚武¹

所属機関： ¹順天堂東京江東高齢者医療センター臨床検査科

ページ範囲：P.1359 - P.1365

フローサイトメトリー(FCM)によるimmunophenotypingは骨髄異形成症候群(MDS)の診断にも利用されているが，その役割は現状では小さなものである．しかし骨髄や末梢血のimmunophenotypingをMDSの診断に利用する試みについては，近年多くの知見が得られており，スコアリングなど手法の進歩もみられ，その価値は変化しつつある．これらの状況について解説し，MDSにおけるimmunophenotypingの標準化についても言及する．

参考文献

1) Brunning RD, Orazi A, Porwit A, et al : Myelodysplastic syndromes. In WHO Classification of tumours of haematopoietic and lymphoid tissues (Swerdlow SH, Campo E, Harris NL, et al, eds), IARC press, Lyon，pp87-107，2008

2) Elghetany MT : Surface marker abnormalities in myelodysplastic syndromes. Haematologica　83：1104-1115，1998

3) Stetler-Stevenson M, Arthur DC, Jabbour N, et al : Diagnostic utility of flow cytometric immunophenotyping in myelodysplastic syndrome. Blood　98：979-987，2001

4) Ogata K, Nakamura K, Yokose N, et al : Clinical significance of phenotypic features of blasts in patients with myelodysplastic syndrome. Blood　100：3887-3896，2002

cells in low-grade myelodysplastic syndromes. Blood　108：1037-1044，2006

6) Pirruccello SJ, Young KH, Aoun P : Myeloblast phenotypic changes in myelodysplasia. CD34 and CD117 expression abnormalities are common. Am J Clin Pathol　125：884-894，2006

7) Xu F, Li X, Wu L, et al : Flow cytometric scoring system (FCMSS) assisted diagnosis of myelodysplastic syndromes (MDS) and the biological significance of FCMSS-based immunophenotypes. Br J Haematol　149：587-597，2010

8) van de Loosdrecht AA, Westers TM, Westra AH, et al : Identification of distinct prognostic subgroups in low- and intermediate-1 -risk myelodysplastic syndromes by flow cytometry. Blood　111：1067-1077，2008

9) Matarraz S, López A, Barrena S, et al : Bone marrow cells from myelodysplastic syndromes show altered immunophenotypic profiles that may contribute to the diagnosis and prognostic stratification of the disease : a pilot study on a series of 56 patients. : Cytometry B Clin Cytom　78：154-168，2010

10) Cherian S, Moore J, Bantly A, et al : Peripheral blood MDS score : a new flow-cytometric tool for the diagnosis of myelodysplastic syndromes. Cytometry B Clin Cytom　64：9-17，2005

11) Cherian S, Moore J, Bantly A, et al : Flow-cytometric analysis of peripheral blood neutrophils : a simple, objective, independent and potentially clinically useful assay to facilitate the diagnosis of myelodysplastic syndromes. Am J Hematol　79：243-245，2005

12) Rashidi HH, Xu X, Wang HY, et al : Utility of peripheral blood flow cytometry in differentiating low grade versus high grade myelodysplastic syndromes (MDS) and in the evaluation of cytopenias. Int J Clin Exp Pathol　5：224-230，2012

13) Sandes AF, Yamamoto M, Matarraz S, et al : Altered immunophenotypic features of peripheral blood platelets in myelodysplastic syndromes. Haematologica　97：895-902，2012

14) Vvan de Loosdrecht AA, Alhan C, Béné MC, et al : Standardization of flow cytometry in myelodysplastic syndromes : report from the European LeukemiaNet working conference on flow cytometry in myelodysplastic syndromes. Haematologica　94：1124-1134，2009

15) Della Porta MG, Picone C, Pascutto C, et al : Multicenter validation of a reproducible flow cytometric score for the diagnosis of low-grade myelodysplastic syndromes : result of a European LeukemiaNET study. Haematologica　97：1209-1217，2012

16) Della Porta MG, Lanza F, Del Vecchio L : Flow cytometry immunophenotyping for the evaluation of bone marrow dysplasia. Cytometry B Clin Cytom　80：201-211，2011