臨床検査57巻4号

2013年04月発行

今月の特集1 次世代の微生物検査

MALDI-TOF MSを利用した菌種同定法

著者： 大楠清文¹

所属機関： ¹岐阜大学大学院医学系研究科病原体制御学分野

ページ範囲：P.388 - P.396

■質量分析は，①試料をイオン化する，②イオンを分離する（重さで分ける），③そのイオンを検出する，の3つのステップからなる．

■MALDI-TOF MSを用いた菌種の同定は，データベースに登録されている菌種とのマススペクトルのパターンマッチングである．

■MALDI-TOF MSは一般細菌だけでなく，嫌気性菌，抗酸菌，酵母様真菌，糸状菌の同定も可能である．さらに，血液培養陽性時の培養液から直接の菌種同定にも活用できる．

参考文献

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18）大楠清文：いま知りたい　臨床微生物検査実践ガイド―珍しい細菌の同定・遺伝検査・質量分析，医歯薬出版，2013