胃と腸44巻2号

2009年02月発行

形態診断に役立つ組織化学・分子生物学

―上皮性腫瘍の粘液細胞形質診断―組織化学：レクチンを含めて（2）

著者： 太田浩良¹ 羽山正義² 小野謙三³ 勝山努⁴

所属機関： ¹信州大学医学部保健学科生体情報検査学 ²信州大学医学部保健学科病因病態検査学 ³公立陶生病院病理部 ⁴信州大学医学部医学科病態解析診断学

ページ範囲：P.275 - P.279

はじめに

　粘液の主体を成すムコ物質はプロテオグリカンと糖蛋白質に大別される．粘液組織化学は，このムコ物質を対象とする組織化学の一分野である．本稿では，粘液の固定法，組織化学的染色の原理とその意義について解説したい．

参考文献

1）Ota H, Katsuyama T.　Alternating laminated array of two types of mucin in the human gastric surface mucous layer. Histochem J 24 : 86-92, 1992

2）Ota H, Hayama M, Momose M, et al. Co-localization of TFF2 with gland mucous cell mucin in gastric mucous cells and in extracellular mucous gel adherent to normal and damaged gastric mucosa. Histochem Cell Biol 126 : 617-625, 2006

3）勝山努，小野謙三．糖質の組織化学．日本病理学会（編）．病理技術マニュアル4 病理組織化学とその技術．医歯薬出版，pp 369-398, 1986

4）Nettleton GS, Carpenter AM. Studies of the mechanism of the periodic acid-Schiff histochemical reaction for glycogen using infrared spectroscopy and model chemical compounds. Stain Technol 52 : 63-77, 1977

5）Spicer SS, Wagner BM, Smith DE. Histochemistry of Connective Tissue. Williams and Wilkins, p251, 1976

6）Katsuyama T, Ono K, Nakayama J, et al. Mucosubstance histochemistry of the normal mucosa and carcinoma of the large intestine. Galactose oxidase-Schiff reaction and lectin stainings. Acta Pathol Jpn 35 : 1409-1425, 1985

7）Ota H, Katsuyama T, Ishii K, et al. A dual staining method for identifying mucins of different gastric epithelial mucous cells. Histochem J 23 : 22-28, 1991

8）Katsuyama T, Spicer SS. Histochemical differentiation of complex carbohydrates with variants of the concanavalin A-horseradish peroxidase method. J Histochem Cytochem 26 : 233-250, 1978

9）Ota H, Hayama M, Nakayama J, et al. Cell lineage specificity of newly raised monoclonal antibodies against gastric mucins in normal, metaplastic and neoplastic human tissues and their application for pathology diagnosis. Am J Clin Pathol 115 : 69-79, 2001

10）Ishihara K, Kurihara M, Goso Y, et al. Peripheralα- linked N-acetylglucosamine on the carbohydrate moiety of mucin derived from mammalian gastric gland mucous cells : epitope recognized by a newly characterized monoclonal antibody. Biochem J 318 : 409-416, 1996

11）Ota H, Nakayama J, Momose M, et al. New monoclonal antibodies against gastric gland mucous cell-type mucins : a comparative immunohistochemical study. Histochem Cell Biol 110 : 113-119, 1998

12）Nakayama J, Yeh JC, Misra AK, et al. Expression cloning of a human alpha1, 4-N-acetylglucosaminyltransferase that forms GlcNAcalpha1―＞4Galbeta―＞R, a glycan specifically expressed in the gastric gland mucous cell-type mucin. Proc Natl Acad Sci USA 96 : 8991-8996, 1999