臨床整形外科47巻1号

2012年01月発行

誌上シンポジウム 整形外科領域における蛍光イメージング

脊髄再生関連　神経幹細胞の蛍光発光標識によるイメージングと応用―脊髄損傷モデルでの移植細胞のBioluminescence Imaging

著者： 高橋勇一朗¹ 岡野栄之² 戸山芳昭¹ 中村雅也¹

所属機関： ¹慶應義塾大学医学部整形外科 ²慶應義塾大学医学部生理学

ページ範囲：P.7 - P.15

　われわれは独自に開発した蛍光・発光蛋白質：ffLucを用いて神経幹細胞を標識することで，移植された細胞を長期にわたり定量的かつ経時的に評価するbioluminescence imagingの系を確立した．これを応用して，脊髄損傷に細胞移植を行ううえで最も適切な移植方法を決めるために，マウス脊髄損傷モデルに対して神経幹細胞を3通りの投与法で移植し，定量的に移植細胞の生着と分布を評価した．移植細胞の生着と安全性の面から，脊髄損傷への神経幹細胞の移植法で最もよい方法は髄内直接投与であることが明らかとなった．蛍光・発光レポーターとその解析法を応用することで，今後さらに脊髄損傷の病態や治療法が解明されることが期待される．

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