臨床整形外科53巻1号

2018年01月発行

誌上シンポジウム 脂肪幹細胞と運動器再生

脂肪由来幹細胞と皮弁生着

著者： 萩原祐介¹ 南野光彦² 橋口宏¹ 園木謙太郎¹ 高井信朗²

所属機関： ¹日本医科大学千葉北総病院整形外科 ²日本医科大学付属病院整形外科

ページ範囲：P.13 - P.20

　脂肪由来幹細胞(ADSCs)の皮弁生着改善作用をin vitroおよびin vivoで評価した．比較対象には骨髄由来幹細胞(BMSCs)を用いた．培養条件下での細胞増殖能，培養7日目以降の血管内皮細胞増殖因子(VEGF)放出能はADSCsが優れていた．培養10日目以降の肝細胞増殖因子(HGF)放出能はBMSCsで多くみられた．ラット背側に作成した皮弁生着距離はADSCs・BMSCs移植群ともにコントロールより有意に延長した．皮弁下血流量の増大が確認され，組織像では移植細胞周囲にVEGFの放出がみられた．

参考文献

1) Lu F, Mizuno H, Uysal CA, et al. Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg 2008;121:50-8.

2) Simman R, Craff C, McKinney B. Improved survival of ischemic random skin flaps through the use of bone marrow nonhematopoietic stem cells and angiogenic growth factors. Ann Plast Surg 2005;54:546-52.

3) Zheng Y, Yi C, Xia W, et al. Mesenchymal stem cells transduced by vascular endothelial growth factor gene for ischemic random skin flaps. Plast Reconstr Surg 2008;121:59-69.

4) Hayashi O, Katsube Y, Hirose M, et al. Comparison of osteogenic ability of rat mesenchymal stem cells from bone marrow, periosteum, and adipose tissue. Calcif Tissue Int 2008;82:238-47.

5) McFarlane RM, DeYoung G, Henry RA. The design of a pedicle flap in the rat to study necrosis and its prevention. Plast Reconstr Surg 1965;35:177-82.

6) Hallock GG. The fallacy of presumed superiority of proximally based versus distally based flaps. Plast Reconstr Surg 1995;96:1372-7.

7) Park S, Tepper OM, Galiano RD, et al. Selective recruitment of endothelial progenitor cells to ischemic tissues with increased neovascularization. Plast Reconstr Surg 2004;113:284-93.

8) Nambu M, Ishihara M, Nakamura S, et al. Enhanced healing of mitomycin C-treated wounds in rats using inbred adipose tissue-derived stromal cells within an atelocollagen matrix. Wound Repair Regen 2007;15:505-10.