BRAIN and NERVE－神経研究の進歩75巻2号

2023年02月発行

総説

カルシウムイメージングによる神経活動の計測

著者： 坂本雅行¹

所属機関： ¹京都大学大学院生命科学研究科光神経分子生理学分野

ページ範囲：P.159 - P.165

文献概要

カルシウムイメージングは現在，ニューロンの発火を計測するイメージング手法として広く用いられている。近年では，高感度かつ高速な遺伝子にコードされたカルシウムセンサーが数多く開発され，多光子励起顕微鏡や内視鏡型顕微鏡を使用することで生体脳においてもニューロンの電気的活動を1細胞レベルの解像度で複数同時に計測することが可能となった。本論では，蛍光カルシウムプローブの特徴や生体脳への応用について紹介する。

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