臨床検査49巻5号

2005年05月発行

今月の主題 マイクロアレイ技術の進歩 総論

DNAマイクロアレイ技術の現状と展望

著者： 郡司渉¹² 村上康文³⁴

所属機関： ¹東京理科大学大学院基礎工学研究科 ²現株式会社バイオマトリックス研究所ゲノム解析事業部 ³東京理科大学基礎工学部生物工学科 ⁴ゲノム創薬研究センター・構造ゲノム科学部門

ページ範囲：P.473 - P.481

〔SUMMARY〕　近年，数多くの遺伝子発現解析に使用されているDNAマイクロアレイであるが，数年前までは再現性や感度，精度が低いことが大きな課題であった．これらの問題点を解決すべく，実験工程の最適化やオリゴヌクレオチドプローブの採用によりDNAマイクロアレイの高性能化が進められ，高感度で高精度な遺伝子発現解析が可能になった．また微量な細胞に由来するRNAの遺伝子発現プロファイルを解析する手法も確立されつつある．今後は，DNAマイクロアレイの解析対象が極めて微量な胚内組織，組織検体，受精卵などに大きく拡がり，臨床や医薬など様々な分野へ一層大きな貢献をするものと考えられる．〔臨床検査　49：473-481，2005〕

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